Comparative Effects of NaCl, PEG and Mannitol Iso-osmotic Stress on Solute Accumulation and Antioxidant Enzyme System in Potato (Solanum tuberosum L.)

نویسندگان

  • Sandip A. Ghuge
  • Archana N. Rai
  • Penna Suprasanna
چکیده

Osmotic and oxidative stress responses to iso-osmotic (-0.4 Mpa) NaCl, PEG-6000 and mannitol stress were studied in 15-day old plants of three potato cultivars viz. ‘Kufri Kufri Bahar’ ‘Jyoti’ and ‘Chandramukhi’. After 2-weeks of treatment, plants were analyzed for biochemical and physiological determinants of stress. Relative water content (RWC) and membrane damage rate (MDR) were significantly affected in all the cultivars in all the treatments. Decrease in RWC was significant in plants subjected to PEG while significant increase in MDR was observed in NaCl-treated plants. Among the treatments, NaCl treatment showed significant MDR (78.57%) over PEG (69.29%) and mannitol (62.90%) treatments. Proline, glycine betaine and total soluble sugar accumulation increased in the stressed plants than controls. Increased SOD activity was observed under NaCl stress compared to PEG/mannitol stress in all the cultivars. Comparison of different iso-osmotic stresses indicates a positive relationship between proline accumulation and tissue hydration. _____________________________________________________________________________________________________________

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تاریخ انتشار 2010